LOCUS       pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N               12842 bp ds-DNA     circular SYN 01-SEP-2022
DEFINITION  rbc L 294 Left_G1397 N.
ACCESSION   .
VERSION     .
KEYWORDS    pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12842)
  TITLE     sTALED for plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2022 from SnapGene Server 1.1.58
            //gt12365.cn
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(5..25)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1857..1922
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5029..5053
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6384..7013
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7442..8515
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8581..8775
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9011..9033)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9119..9259
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9174..9193
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9274..9291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9445..10033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9525..9544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10279..11070)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    11591..11615
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      11708..11960
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11980..12531)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12573..12752)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cgttcaactt ttctatacaa agttgaagat ctgactcaac ttttgattcg ctatttgcag
       61 tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca ttgctattac
      121 aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca taaccatttt
      181 ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac cagctccgtt
      241 tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc tttgattgat
      301 cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa acattgcatt
      361 cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag ctttctacat
      421 gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa gcacgaacac
      481 cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg ttgcgatatt
      541 gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt aacaatgaac
      601 aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca ggcttaattt
      661 tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag ataatgcaca
      721 agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt cattaatctc
      781 taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa actacaccaa
      841 ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg gagtgtatat
      901 ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata ttatcggttt
      961 atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct ttcaatttca
     1021 tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta gcttggagtc
     1081 aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt ttatatcttg
     1141 caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga tcactgactt
     1201 ggttctaacc aaaaaaaaaa aaatgtttaa tttacatatc taaaagtagg tttagggaaa
     1261 cctaaacagt aaaatatttg tatattattc gaatttcact catcataaaa acttaaattg
     1321 caccataaaa ttttgtttta ctattaatga tgtaatttgt gtaacttaag ataaaaataa
     1381 tattccgtaa gttaaccggc taaaaccacg tataaaccag ggaacctgtt aaaccggttc
     1441 tttactggat aaagaaatga aagcccatgt agacagctcc attagagccc aaaccctaaa
     1501 tttctcatct atataaaagg agtgacatta gggtttttgt tcgtcctctt aaagcttctc
     1561 gttttctctg ccgtctctct cattcgcgcg acgcaaacga tcttcaggtg atcttctttc
     1621 tccaaatcct ctctcataac tctgatttcg tacttgtgta tttgagctca cgctctgttt
     1681 ctctcaccac agccatggat tcacagctag tcttgtctct gaagctgaat ccaagcttca
     1741 ctcctctttc tcctctcttc cctttcactc catgttcttc tttttcgccg tcgctccggt
     1801 tttcttcttg ctactcccgc cgcctctatt ctccggttac cgtctacgcc gcgaaggact
     1861 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     1921 agcccgggga tctacgcacg ctcggctaca gccagcagca acaggagaag atcaaaccga
     1981 aggttcgttc gacagtggcg cagcaccacg aggcactggt cggccatggg tttacacacg
     2041 cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct gtcaagtatc
     2101 aggacatgat cgcagcgttg ccagaggcga cacacgaagc gatcgttggc gtcggcaaac
     2161 agtggtccgg cgcacgcgct ctggaggcct tgctcacggt ggcgggagag ttgagaggtc
     2221 caccgttaca gttggacaca ggccaacttc tcaagattgc aaaacgtggc ggcgtgaccg
     2281 cagtggaggc agtgcatgca tggcgcaatg cactgacggg tgcccccctg aacctcacac
     2341 cagatcaggt ggtggccatt gcaagcaata atggtggcaa gcaagccctc gaaaccgtcc
     2401 aaaggctcct tcctgtcttg tgtcaggcac atggcctcac tccagaccag gtggtggcaa
     2461 tcgcttctca tgatggagga aaacaagcac ttgagactgt tcagcggctt ttgcctgttc
     2521 tttgccaaga ccatggactt accccagacc aggtcgttgc catcgcctca catgacggtg
     2581 gaaagcaggc cttggaaaca gtgcaacgct tgctccccgt tctgtgccaa gctcatgggt
     2641 tgactcctga tcaagtggtt gccattgcct ctaacaacgg cggaaaacag gctcttgaga
     2701 ctgtccagag gctgctgccc gtgctctgtc aggatcatgg actcacacct gctcaagtgg
     2761 ttgcaatcgc cagcaatatt ggcggtaaac aggccttgga aacagttcaa cgcttgcttc
     2821 cagtcttgtg ccaagctcac gggctgaccc cagagcaggt cgtggctatc gcatctaaca
     2881 atggtggtaa gcaagctctg gagacagtgc agagacttct cccagttctg tgccaggccc
     2941 acggcctcac accagcacaa gtggtggcta tcgctagtaa cattggtggt aaacaagctc
     3001 tggaaaccgt tcagcgcttg ctccctgtgc tgtgtcaaga tcacggattg acccctgccc
     3061 aagtggtggc aatcgcttct aatgggggcg ggaagcaagc cctggagact gtgcagagac
     3121 ttttgcccgt tctttgccag gatcatgggc tcaccccaga tcaggtcgtc gcaatcgctt
     3181 ccaacatagg aggtaagcag gcactcgaaa cagttcaacg gctcctcccc gtgttgtgcc
     3241 aagctcacgg tttgacccct gaacaagtcg tggctattgc tagtaacatc ggtggtaaac
     3301 aggctcttga gacagttcaa agattgctgc cagtgttgtg tcaggctcac ggtttgactc
     3361 cagctcaagt tgttgcaatc gccagtaacg gtggggggaa gcaggcactc gaaaccgtgc
     3421 aacggcttct ccccgttctg tgccaggcac acggactgac accagcccaa gttgtcgcca
     3481 tcgcttcaaa taacgggggt aaacaagctc tcgagacagt gcaacgcttg ttgcctgtgc
     3541 tttgccaaga tcatgggttg actccagatc aggtggtggc cattgcaagc aataatggtg
     3601 gcaagcaagc cctcgaaacc gtccaaaggc tccttcctgt cttgtgtcag gcacatggcc
     3661 tcactccaga ccaggtggtg gcaatcgctt ctcatgatgg aggaaaacaa gcacttgaga
     3721 ctgttcagcg gcttttgcct gttctttgcc aagaccatgg acttacccca gaccaggtcg
     3781 ttgccatcgc ctcacatgac ggtggaaagc aggccttgga aacagtgcaa cgcttgctcc
     3841 ccgttctgtg ccaagctcat ggattgaccc ctgaacaggt tgttgctatc gcatcaaatg
     3901 gtggaggcaa gcaagctttg gaaaccgttc agcggctttt gccagtcctc tgtcaggcac
     3961 acgggctcac accagatcag gtcgtcgcca tcgccagtaa tattggggga aagcaagcac
     4021 ttgaaacagt gcaaagattg ttgcccgtgt tgtgccaagc acatggactc actcccgcac
     4081 aagtggtcgc aattgccagc cacgacggcg ggaaacaagc cctcgaaact gtgcagaggc
     4141 tcctccctgt cctctgtcaa gatcacggtc tgactccgga acaggtggtg gcgattgcaa
     4201 gcaacggcgg cggcaaacag gctctagaga gcattgttgc ccagctctcc agacctgatc
     4261 cggcgctagc cgcgttgaca aatgatcatc ttgttgctct tgcttgtctt ggcggacgac
     4321 ctgccctgga tgctgtgaaa aaggggttgg ggggatccgg atcaggtagc tacgcacttg
     4381 gtccttacca gattagcgca ccccaactcc ccgcctataa tggtcaaacc gtcgggacct
     4441 tttactacgt aaacgatgct ggtgggctgg aatccaaagt attctcctca gggggcccta
     4501 caccctaccc caactacgcc aatgctggtc atgtagaagg gcagtcagca ctgtttatgc
     4561 gcgataatgg tataagcgag gggttggtct tccataacaa cccagagggt acttgtggct
     4621 tctgtgtgaa tatgactgaa acccttctgc ccgaaaatgc caagatgact gtcgtcccac
     4681 ctgaaggcta gggtacctga taatcgatat cccgcggcca tgctagagtc cgcaaaaatc
     4741 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
     4801 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     4861 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     4921 aaaccaaaat ccagtgacct gcaggcatgc gacgtccgtc gggcccaagc ttagcttgag
     4981 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     5041 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     5101 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     5161 ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc aacccctccg
     5221 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     5281 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     5341 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     5401 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     5461 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     5521 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     5581 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     5641 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     5701 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     5761 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     5821 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     5881 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     5941 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     6001 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     6061 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     6121 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     6181 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     6241 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     6301 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     6361 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     6421 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     6481 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     6541 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     6601 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     6661 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     6721 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     6781 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     6841 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     6901 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     6961 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     7021 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     7081 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     7141 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     7201 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     7261 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     7321 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     7381 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     7441 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     7501 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     7561 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     7621 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     7681 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     7741 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     7801 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     7861 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     7921 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     7981 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     8041 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     8101 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     8161 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     8221 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     8281 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     8341 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     8401 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     8461 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     8521 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     8581 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     8641 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     8701 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     8761 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     8821 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     8881 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     8941 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     9001 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     9061 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     9121 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     9181 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     9241 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10081 aaactcacgt taagggattt tggtcatgca tgatatatct cccaatttgt gtagggctta
    10141 ttatgcacgc ttaaaaataa taaaagcaga cttgacctga tagtttggct gtgagcaatt
    10201 atgtgcttag tgcatctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa
    10261 cgaatttcta gctagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
    10321 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
    10381 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
    10441 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
    10501 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
    10561 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
    10621 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
    10681 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
    10741 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
    10801 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
    10861 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
    10921 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
    10981 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
    11041 cgatacttcg gcgatcaccg cttcccccat gatgtttaac tttgttttag ggcgactgcc
    11101 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
    11161 ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat
    11221 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
    11281 tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt
    11341 tcgtgcccga attgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
    11401 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
    11461 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
    11521 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    11581 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
    11641 acacattgcg gacgttttta atgtactgaa ttaacgccga attgaattat cagcttgcat
    11701 gccggtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
    11761 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
    11821 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
    11881 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
    11941 tgccaaatgt ttgaacgatc tgcttgactc taggggtcat cagatttcgg tgacgggcag
    12001 gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca
    12061 gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc
    12121 gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc
    12181 ctgtgcctcc agggacttca gaaggtgggt gtagagcgtg gagcccagtc ccgtccgctg
    12241 gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt
    12301 ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg
    12361 atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt
    12421 acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat
    12481 gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc
    12541 cctcgatcga gttgagagtg aatatgagac tctaattgga taccgagggg aatttatgga
    12601 acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact
    12661 tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc
    12721 gcggctcagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt
    12781 cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcgataa
    12841 tt
//